Author: sysbioweb

What is Eau de Toilette (EDT) ?

Eau de Toilette (EDT) is a type of fragrance that has a lower concentration of fragrance oils compared to Eau de Parfum (EDP) and perfume. Eau de Toilette offers a lighter and more refreshing scent experience, making it suitable for everyday wear and various occasions.

Typically, Eau de Toilette contains a fragrance oil concentration ranging from 5% to 15%, although the exact percentage can vary between brands and perfumes. The lower concentration of oils in Eau de Toilette results in a lighter and more subtle fragrance compared to Eau de Parfum.

Eau de Toilette provides a pleasant and moderate scent that is suitable for casual or daytime use. It has a good balance between fragrance intensity and longevity. On average, Eau de Toilette lasts between 2 to 4 hours on the skin, although the duration can vary based on individual factors such as skin chemistry and environmental conditions.

The lighter nature of Eau de Toilette makes it a popular choice for those who prefer a more understated or less overpowering fragrance. It is often favored for office environments, warmer climates, or situations where a softer scent presence is desired.

Eau de Toilette offers a good projection, meaning the fragrance subtly emanates from the skin, creating a pleasant scent aura around the wearer. The moderate concentration allows for a balanced distribution of the fragrance notes, providing a harmonious and enjoyable scent experience.

Eau de Toilette is widely available and is typically packaged in spray bottles or atomizers for easy application. Its versatility and lighter scent make it suitable for regular use and frequent reapplication throughout the day if desired.

While Eau de Toilette may not have the same longevity as perfume or Eau de Parfum, it offers a pleasant and approachable fragrance option that can be enjoyed by a wide range of individuals.

What is Eau de Parfum (EDP) ?

Eau de Parfum (EDP) is a type of fragrance that has a slightly lower concentration of fragrance oils compared to perfume (Parfum or Extrait de Parfum). Eau de Parfum is known for its balance between fragrance intensity and longevity, making it a popular choice among perfume enthusiasts.

Typically, Eau de Parfum contains a fragrance oil concentration ranging from 10% to 20%, although the exact percentage can vary between brands and perfumes. It is a step down in concentration from perfume but still offers a significant presence and longevity on the skin.

Eau de Parfum provides a noticeable and long-lasting scent experience. It generally lasts between 4 to 6 hours on the skin, although the duration can be influenced by factors such as individual skin chemistry, application technique, and environmental conditions.

Compared to perfume, Eau de Parfum offers a slightly lighter and more wearable option. It has a good balance between the intensity of the fragrance and the ability to be worn in various settings. Eau de Parfum can be suitable for both day and evening wear, depending on the specific fragrance and personal preference.

Due to its moderate concentration, Eau de Parfum allows for a broader distribution of the fragrance notes, providing a pleasant and well-rounded scent experience. It typically has a good projection, meaning the fragrance can radiate from the skin and create a scented aura around the wearer.

Eau de Parfum is widely available in the fragrance market and is often packaged in spray bottles or atomizers for easy application. It offers a desirable combination of longevity, intensity, and versatility, making it a popular choice for those seeking a lasting and impactful fragrance.

What is Perfume (Parfum or Extrait de Parfum)

Perfume, also known as Parfum or Extrait de Parfum, refers to the highest concentration of fragrance in a perfume product. It contains the most concentrated form of perfume oils, making it the most potent and long-lasting type of fragrance available.

Perfume typically has a fragrance oil concentration ranging from 15% to 30%, although the exact concentration may vary between brands and perfumes. It contains a high proportion of pure perfume essence, which is mixed with a base of alcohol or a carrier oil to create the final product.

Due to its high concentration, perfume is known for its luxurious and intense nature. It often requires only a small amount to create a long-lasting and noticeable fragrance on the skin. Perfume has a strong scent projection and can linger for many hours, typically lasting around 6 to 8 hours or even longer, depending on factors such as individual skin chemistry and environmental conditions.

Perfume is often considered the most expensive and prestigious form of fragrance due to its high concentration of fragrance oils. It is typically packaged in small bottles or vials and is meant to be applied sparingly to specific pulse points on the body for optimal diffusion and longevity.

While perfume is highly concentrated and long-lasting, it may not be suitable for those who prefer a more subtle or lighter fragrance. Other concentrations such as Eau de Parfum, Eau de Toilette, or Eau de Cologne offer lighter options for those who desire a more understated scent or prefer a different balance between fragrance intensity and longevity.

What are the different types of perfume?

Perfumes can be categorized into different types based on their concentration of fragrance oils. The concentration of oils determines the intensity and longevity of the fragrance. Here are the main types of perfumes based on concentration, listed in descending order of oil concentration:

Perfume (Parfum or Extrait de Parfum): Perfume has the highest concentration of fragrance oils, usually ranging from 15% to 30%. It contains a high proportion of pure perfume essence and is known for its luxurious and long-lasting nature. Perfume typically lasts for 6 to 8 hours or even longer on the skin.

Eau de Parfum (EDP): Eau de Parfum has a slightly lower concentration of fragrance oils compared to perfume, typically ranging from 10% to 20%. It is still a relatively potent and long-lasting fragrance option. Eau de Parfum can last between 4 to 6 hours on the skin.

Eau de Toilette (EDT): Eau de Toilette has a lower concentration of fragrance oils, usually ranging from 5% to 15%. It is lighter and more suitable for everyday wear. Eau de Toilette typically lasts between 2 to 4 hours on the skin.

Eau de Cologne (EDC): Eau de Cologne has a lower concentration of fragrance oils compared to the above types, usually ranging from 2% to 4%. It is often fresh and citrusy in nature. Eau de Cologne has a shorter longevity, usually lasting around 2 hours or less on the skin.

Splash or Aftershave: Splashes and aftershaves have the lowest concentration of fragrance oils, usually below 2%. They are often used for a quick refreshing effect and may not last very long on the skin. It’s important to note that the actual concentration of fragrance oils may vary between brands and perfumes, and there can be overlap between these categories. Additionally, the longevity of a perfume can also be influenced by other factors such as individual skin chemistry and environmental conditions.

Comparative modelling and structure based drug repurposing of PAX2 transcription factor for targeting acquired chemoresistance in pancreatic ductal adenocarcinoma

Pancreatic ductal adenocarcinoma (PDAC) is a pancreatic malignancy suffering from poor prognosis; the worst among all types of cancer. Chemotherapy, which is the standard regime for treatment in most cases, is often rendered useless as drug resistance quickly sets in after prolonged exposure to the drug. The implication of PAX2 transcription factor in regulating several ATP-binding cassette (ABC) transporter proteins that are responsible for the acquisition of drug resistance in PDAC makes it a potential target for treatment purposes. In this study, the 3D structure of PAX2 protein was modelled, and the response of key amino acids to perturbation were identified. Subsequently, kappadione, a vitamin K derivative, was found to bind efficiently to PAX2 with a binding energy of -9.819 kcal/mol. The efficacy of mechanism and mode of binding was studied by docking the protein with DNA in the presence and absence of the drug. The presence of kappadione disrupted DNA binding with key effector resides, preventing the DNA from coming into contact with the binding region essential for protein translation. By occupying the DNA binding region and replacing it with a ligand, the mechanism by which DNA interacts with PAX2 could be manipulated. Inhibition of PAX2-DNA binding using kappadione and other small molecules can prove to be beneficial for combating chemoresistance in PDAC, as proposed through in silico approaches. DOI:  https://doi.org/10.1080/07391102.2020.1742793

DeepOlf: Deep neural network based architecture for predicting odorants and their interacting Olfactory Receptors

Abstract:

Olfaction transduction mechanism is triggered by the binding of odorants to the specific olfactory receptors (OR’s) present in the nasal cavity. Different odorants stimulate different OR’s due to the difference in shape, physical and chemical properties. In this paper, a deep neural network architecture DeepOlf, based on molecular features and fingerprints of odorants and ORs, to predict whether a chemical compound is a potential odorant or not along with its interacting OR is proposed. Odorant identification and Odorant-OR interaction were modeled as a binary classification through multiple classifiers. The evaluation of these classifier’s performance showed that the deep-neural network framework not only fits data with better accuracy in comparison to other classical methods (SVM, RF, k-NN) but also able to predict odorant-OR interactions more accurately. To our knowledge, this study is the first realization of deep learning ideas for the problem of odorant and interacting OR prediction. The accuracy of DeepOlf was found to be 94.83% and 99.92 % for the prediction of odorants and Odorant- OR interactions respectively. Comparison of DeepOlf prediction with the existing SVM based prediction server, ODORactor, showed that better performance can be achieved with the proposed deep learning approach. The DeepOlf tool can be accessed at https://bioserver.iiita.ac.in/deepolf/.
Published in: IEEE/ACM Transactions on Computational Biology and Bioinformatics ( Early Access )
Date of Publication: 12 June 2020 

DeEPn: A deep neural network based tool for enzyme functional annotation

Authors: Rahul Semwal, Imlimaong Aier, Pankaj Tyagi, Pritish Kumar Varadwaj
With the advancement of high throughput techniques, the discovery rate of enzyme sequences has increased significantly in the recent past. All of these raw sequences are required to be precisely mapped to their respective functional attributes, which helps in deciphering their biological role. In the recent past, various prediction models have been proposed to predict the enzyme functional class; however, all of these models were able to quantify at most six functional enzyme classes (EC1 to EC6) out of existing seven functional classes, making these approaches inappropriate for handling enzymes corresponding to the seventh functional class (EC7). In this study, a Deep Neural Network-based approach, DeEPn, has been proposed, which can quantify enzymes corresponding to all seven functional classes with high precision and accuracy. The proposed model was compared with two recently developed tools, ECPred and SVM-Prot. The result demonstrated that DeEPn outperformed ECPred and SVM-Prot in terms of predictive quality. The DeEPn tool has been hosted as a web-based tool at https://bioserver.iiita.ac.in/DeEPn/.

Understanding Drug Resistance Mechanism of NS3/4A Protease of HCV Using Comparative Molecular Dynamics Simulation

Hepatitis C virus (HCV) infects approximately 325 million individuals globally causing hepatitis C, a fatal disease leading to liver cirrhosis. Imperative sincere efforts are needed to develop inhibitors targeting the essential NS3/4A protease. NS3/4A protease is an exceptionally significant target. Resistance against the most promising protease inhibitors, Telaprevir, Boceprevir and Faldaprevir has emerged in clinical trials. The emergence of resistance is attributed to the error-prone viral RNA-dependent RNA polymerase, thereby reducing the effectiveness of these inhibitors. Among the drug-resistant variants, single amino acid residues (V35M, Q80K, R155K, A156V, and D168A) are noteworthy for their presence in clinical isolates and also their efficacy against these inhibitors in clinical development. Thus, it is essential to unravel the mechanistic insights of these drug-resistant variants while designing potent novel inhibitors. In this current work, we have performed molecular docking and comparative MD simulation to analyze and unravel molecular mechanism of conformational fluctuations among inhibitor binding between wild type and its V35M, Q80K, R155K, A156V, and D168A variants. Protein-ligand contacts, Root mean square deviation (RMSD), Root mean square fluctuation(RMSF) and post-simulation plot analysis has been used to identify the stability and conformation of the key residues that regulate inhibitor binding and their impact in developing drug resistance. Unraveling and understanding of the binding mechanism of inhibitor within substrate would be a significant approach to design inhibitor that fits within the substrate with less susceptibility towards drug resistance as mutations upsetting inhibitor binding would concurrently impede the recognition of viral substrates.

Targeting INSM1 in Order to Prevent the Growth of Small Cell Lung Cancer

Small cell lung cancer (SCLC) is a very aggressive form of cancer because of its poor survival and high rate of tumour progression. There are very few commercial drugs that can specifically target SCLC tumours and inhibit their progression. Thus, there is an urgent and unmet need to identify novel and effective drugs that can specifically target the SCLC tumour progression. In this study, we have selected Insulinoma associated protein 1 (INSM1), a transcription factor responsible for neuro-endocrinal differentiation. It has also been studied as a marker for neuro-endocrinal tumours like SCLC. It also plays a role in activation of several key pathways that are responsible for SCLC progression. N-myc which is an intermediate of the Sonic Hedgehog (Shh) pathway, helps in the overexpression of INSM1 which in turn activates PI3K/AKT and MEK/ERK1/2 pathways. The activation of these pathways results in further stabilization of N-myc and this cycle repeats. Thus, INSM1 acts as a key intermediate molecule between the Shh, PI3K/AKT and MEK/ERK1/2 pathways which have been reported to play an important role in tumour progression. It has already been reported that the knockdown of INSM1 can significantly reduce the SCLC tumour progression. Thus, we have screened the target INSM1 against our library of plant based anti-cancerous compounds to obtain a suitable drug. The top compounds having better docking score were studied for their ADME properties. The best compound obtained is then simulated for 50 ns in order to study the stability of the protein-ligand interaction. From this study, we try to identify novel plant-based therapeutics to inhibit SCLC tumour progression by inhibiting INSM1.

Understanding the Mechanism of Cell Death in Gemcitabine Resistant Pancreatic Ductal Adenocarcinoma: A Systems Biology Approach

Background: Gemcitabine is the standard chemotherapeutic drug administered in advanced Pancreatic Ductal Adenocarcinoma (PDAC). However, due to drug resistance in PDAC patients, this treatment has become less effective. Over the years, clinical trials for the quest of finding novel compounds that can be used in combination with gemcitabine have met very little success.

Objective: To predict the driving factors behind pancreatic ductal adenocarcinoma, and to understand the effect of these components in the progression of the disease and their contribution to cell growth and proliferation.

Methods: With the help of systems biology approaches and using gene expression data, which is generally found in abundance, dysregulated elements in key signalling pathways were predicted. Prominent dysregulated elements were integrated into a model to simulate and study the effect of gemcitabine- induced hypoxia.

Results: In this study, several transcription factors in the form of key drivers of cancer-related genes were predicted with the help of CARNIVAL, and the effect of gemcitabine-induced hypoxia on the apoptosis pathway was shown to have an effect on the downstream elements of two primary pathway models; EGF/VEGF and TNF signalling pathway.

Conclusion: It was observed that EGF/VEGF signalling pathway played a major role in inducing drug resistance through cell growth, proliferation, and avoiding cell death. Targeting the major upstream components of this pathway could potentially lead to successful treatment.